In this answer, purity of ghee is tested by comparing home made ghee with store bought ghee. Store bought ghee is adulterated with three main ingredients. Learn about adulteration of ghee, types of adulterants, detection of adulterants in ghee. Buy unadulterated Pure Desi Ghee at VedicGiftShop. Detection of adulteration of ghee (clarified milk fat) with palm olein and sheep body fat using Reichert-Meissl (RM) value coupled with solvent.

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J Nat Sci Biol Med. The composition of ghee is Evaluation of fast gas adultration and gas chromatography-mass spectrometry in the analysis of lipids. Not just in cooking, ghee has also been an important ingredient in many Ayurvedic medicines.

Mix thoroughly and allow to stand for 10 min. Although some people disagree, most nutritionists, doctors and health professionals believe that ghee in reasonable amounts is good for health.

Now you have pure ghee ready. Thus, it is said to promote positivity, growth and expansion of the consciousness and detoxify the body. The data reveals that there are clear differences in the second order derivative spectra of adultfration and cow ghee.

In another test tube mix aduteration. Leave a Reply Cancel reply. After collecting the cream for a few days, churn it in a mixer, which separates the butter. Mix the melted ghee with the furfural acid solution. Correlation of tallow concentration in ghee with the ratio of absorbance.

There are no reliable tests that can be conducted at home for this type of adulteration. Table 1 Details of second order derivative spectra of ghee and tallow samples.

It contains a good amount of beta-carotene and vitamin E which are antioxidants. If crimson colour appears, add 10 ml of water shake and allow to stand for another 10 min. Support Center Support Center. The study reports a rapid, sensitive, cost-effective derivative spectrophotometric method for detection of tallow adulteration in cow ghee.


Cambridge University Press; The detection of ghee with vanaspati be detect by using following tow tests. Sample preparation One gram of pure ghee and tallow were taken separately, melted by warming, 10 ml chloroform analytical grade from Merck India adultwration added, sonicated for 15 min to make it homogenous and bhee used as stock solution.

If, after some time, the bottom layer shows pink or red colour, the sample is adulterated with rancid ghee.

Mixture of ghee and tallow in different proportions ranging from 9: If the sample is adulterated, the ghee and the coconut ghew will solidify in separate layers. Test tube stand Procedure: The normal UV spectra of cow ghee and tallow [ Figure 1 ] did not reveal sufficient information that can be useful for detection of tallow adulteration in cow ghee.

In rare cases, ghee may be adulterated with animal fat like tallow. It also contains other fat-soluble vitamins such as vitamin D and K2, which are usually lacking in the diet otherwise, and many of the minerals that are needed by our bodies.

Cow ghee, second order jn, tallow adulteration, ultraviolet spectrophotometry. To make ghee, the fat ih cream from the milk can be skimmed off after the milk is boiled and cooled.

Unlike other oils, ghee is rich in butyric acid. Adulteration is, therefore, a real threat. Tests that can be done at home There are several tests that can be done at wdulteration to test the purity of ghee. The naturally occurring butyric acid in ghee can help in the treatment of these diseases. These samples were used for the study.

Detection of tallow adulteration in cow ghee by derivative spectrophotometry

Ghee or clarified butter has been an integral part of Indian adulteratoin from time immemorial. Qdulteration such as ultraviolet UV fluorescence technique,[ 8 ] paper chromatography,[ 910 ] gas chromatography GC ,[ 11 ] GC-mass,[ 12 ] combination of preparative thin layer chromatography and GC[ 13 ] are reported for detection of tallow adulteration in ghee. Hence, it will be useful in detecting and quantifying adulteration of tallow in cow ghee.


In recent years, however, research seems to indicate that ghee is better than many of the seed and vegetable oils that were used in its stead.

Detection of Adulteration in Ghee

Take ml of melted ghee in a test tube. Add 5ml of 0. UV spectrophotometry The normal absorption spectra of ghee and tallow of all the samples were recorded in the range nm using a PerkinElmer UV-visible recording spectrometer Model — Lambda Melt a small quantity of ghee in a vessel. Evaluation must be efficient in evaluating the quality of ghee on the basis of physical characteristic. An on-line differential converter, for obtaining the first derivative of absorption spectra. Woodhead Publishing Limited; Butyric acid in the gut supports the production of T-cells which are the cells that fight infections and bacteria.

If the iodine solution, which is brown in colour, turns purple, then the ghee is adulterated with a starch like mashed potato or sweet potato.

One gram of pure ghee and tallow were taken separately, melted by warming, 10 ml chloroform analytical grade from Merck India was added, sonicated for 15 min to make it homogenous and were used as stock solution. Melt a small quantity of the ghee and pour into the glass jar. Consuming ghee, therefore, helps in building a strong immune system. This decrease shows consistent linearity suggesting its potential applicability for quantitative estimation of tallow in cow ghee.

Along with being rich in vitamins A, E and K, ghee also contains omega 3, 6 and 9 fatty acids which are good for the functioning of the heart and brain. Take about one teaspoonful of melted ghee in the test tube or bottle. Muthanna MC, Mukherji B.